Autoimmune Signaturen des Alterns und altersbedingter neurodegenerativer Erkrankungen stehen im Zusammenhang mit der Gehirnfunktion und ribosomalen Proteinen
Die biologische Alterung ist ein komplexer Prozess, der durch eine verminderte Funktion von Zellen und Geweben, einschließlich derjenigen des Immunsystems, gekennzeichnet ist. Folglich beeinflusst das Altern die Expression und Entwicklung von Autoantikörpern und autoreaktiven T-Zellen, die in ihrer Summe als Autoimmunom eines Individuums gesehen werden können. In dieser Studie haben wir analysiert, ob Sätze von Autoimmunmerkmalen mit spezifischen Phänotypen assoziiert sind, die autoimmune Signaturen im Zusammenhang mit Alter und neurodegenerativen Erkrankungen bilden. Die Autoantikörperprofildaten von gesunden Probanden und Patienten aus der GEO-Datenbank wurden verwendet, um autoimmune Signaturen des Alterns und dreier neurodegenerativer Erkrankungen, darunter Parkinson-Krankheit (PD), Alzheimer-Krankheit (AD) und A number of Sklerose (MS), zu untersuchen.
Unsere Ergebnisse zeigen, dass die autoimmune Signatur des Alterns durch einen wellenförmigen Anstieg von IgG-Autoantikörpern im Zusammenhang mit Lernen und Verhalten und einem konsistenten Anstieg von IgG-Autoantikörpern im Zusammenhang mit Ribosomen und Translation gekennzeichnet ist Krankheiten. Interessanterweise identifizierte die Differential Expression-Sliding Window Evaluation (DE-SWAN) drei Wellen von Veränderungen von Autoantikörpern während des Alterns im Alter von 30, 50 bzw. 62 Jahren. Darüber hinaus sind insbesondere IgG-Autoantikörpersolche gegen ribosomale Proteine, könnten als Vorhersagemarker für das Altern und altersbedingte neurodegenerative Erkrankungen verwendet werden. Daher deckt diese Studie erstmals umfassende autoimmunologische Signaturen für das Altern und altersbedingte neurodegenerative Erkrankungen auf.
Kombination von schnellem mikrofluidischem Mischen und Dreifarben-Einzelmolekül-FRET zur Untersuchung der Kinetik von Protein- Konformationsänderungen
Der Einzelmolekül-Förster-Resonanzenergietransfer (FRET) ist aufgrund seiner hohen Empfindlichkeit und Fähigkeit, einzelne Subpopulationen in heterogenen Systemen aufzulösen, intestine geeignet, um die Kinetik von Protein-Konformationsänderungen zu untersuchen. Der gebräuchlichste Ansatz, der zwei Fluorophore verwendet, kann jedoch jeweils nur eine Distanz überwachen, und die Verwendung von drei Fluorophoren zum gleichzeitigen Überwachen mehrerer Distanzen ist weitgehend auf Gleichgewichtsschwankungen beschränkt. Hier zeigen wir, dass Dreifarben-Einzelmolekül- FRET mit schnellem mikrofluidischem Mischen kombiniert werden kann , um Konformationsänderungen in einem Protein von Millisekunden bis Minuten zu untersuchen. In Kombination mit manuellem Mischen haben wir die Kinetik auf 1 h verlängert, was einem Gesamtzeitbereich von 5 Größenordnungen entspricht.
Wir untersuchten die Umwandlung von Monomer zu Protomer des porenbildenden Toxins Cytolysin A (ClyA), einem der größten bekannten Konformationsübergänge von Proteinen. Die ortsspezifische Markierung von ClyA mit drei Fluorophoren ermöglichte es uns, die Kinetik von drei intramolekularen Abständen gleichzeitig zu verfolgen und zeigte ein bisher unentdecktes Intermediat. Die Kombination von dreifarbigem Einzelmolekül- FRET mit schneller mikrofluidischer Mischung bietet somit einen Ansatz, um die Mechanismen komplexer biomolekularer Prozesse mit hoher Zeitauflösung zu untersuchen.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Rabbit Polyclonal CD31 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.
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Krebsimmuntherapie durch NC410, ein LAIR-2 Fc- Protein, das die menschliche LAIR-Kollagen-Interaktion blockiert
Kollagene sind ein Hauptbestandteil der extrazellulären Matrix und sind funktionelle Liganden für den inhibitorischen Immunrezeptor- Leukozyten-assoziierten Immunglobulin-ähnlichen Rezeptor (LAIR)-1. LAIR-2 ist ein sezerniertes Protein, das als Köderrezeptor fungieren kann, indem es Kollagen mit einer höheren Affinität als LAIR-1 bindet. Wir schlagen vor, dass Kollagene die Immunevasion durch Interaktion mit LAIR-1 fördern, das auf Immunzellen exprimiert wird, und dass LAIR-2 eine LAIR-1-vermittelte Immunsuppression freisetzt.
Die Analyse öffentlicher Humandatensätze zeigt, dass Kollagene, LAIR-1 und LAIR-2 einzigartige und sich überschneidende Assoziationen mit dem Überleben in bestimmten Tumoren aufweisen. Wir entwarfen ein dimeres LAIR-2 mit einem funktionellen IgG1-Fc-Schwanz, NC410, und zeigten, dass NC410 die Enlargement von humanen T- Zellen und die Effektorfunktion in vivo in einem xenogenen Graft-versus-Host-Krankheitsmodell der Maus erhöht. In humanisierten Maus-Tumormodellen reduziert NC410 das Tumorwachstum, das von T-Zellen abhängig ist. Die immunhistochemische Analyse von menschlichen Tumoren zeigt, dass NC410 an kollagenreiche Bereiche bindet, in denen LAIR-1 + -Immunzellen lokalisiert sind . Unsere Ergebnisse zeigen, dass NC410 eine neue Strategie für die Krebsimmuntherapie bei immunausgeschlossenen Tumoren sein könnte.
Immunologische Identifizierung und Charakterisierung des Capsid Scaffold Protein kodiert durch UL26.5 von Herpes – simplex – Virus Typ 2
Herpes-simplex-Virus Typ 2 (HSV2), ein Erreger, der Genitalherpes-Läsionen verursacht, greift über verschiedene bekannte und unbekannte Mechanismen in das Immunsystem des Wirts ein . Dieses Virus wurde verwendet, um die virale Antigenzusammensetzung zu untersuchen. Rekonvaleszenzserum von HSV2-infizierten Patienten wurde verwendet, um virale Antigene mittels 2D- Proteinelektrophorese und Immunblotting zu identifizieren . Das Serum erkannte überwiegend mehrere Kapsidgerüstproteine, die vom Gen UL26.5, hauptsächlich ICP35, kodiert wurden .
Von diesem Protein wurde hauptsächlich berichtet, dass es vorübergehend beim viralen Zusammenbau funktioniert, aber nicht in reifen Viruspartikeln exprimiert wird. Weitere immunologische Studien legten nahe, dass dieses Protein bei Mäusen spezifische Antikörper- und zytotoxische T-Lymphozyten (CTL) -Reaktionen hervorruft, aber diese Reaktionen führen nicht zu einer klinischen Schutzwirkung als Reaktion auf eine HSV2-Provokation . Die Daten legten nahe, dass die Immundominanz von ICP35 verwendet werden könnte, um ein integriertes Antigen mit anderen viralen Glykoproteinen zu entwickeln.
Identifizierung von 7.000-9.000 Proteinen aus Zelllinien und Geweben durch Single-Shot Microflow LC-MS/MS
Ein aktueller Pattern in der Proteomik besteht darin, Daten in einem „Single-Shot“ mit LC-MS/MS zu erfassen, da dies die Arbeitsabläufe vereinfacht und einen besseren Durchsatz und eine höhere quantitative Genauigkeit verspricht als Schemata, die eine umfangreiche Probenfraktionierung beinhalten. Single-Shot-Ansätze können jedoch unter einer begrenzten Proteomabdeckung leiden, wenn sie durch datenabhängige Erfassung (ssDDA) auf Nanoflow-LC-Systemen durchgeführt werden. Für Anwendungen, bei denen Probenmengen nicht knapp sind, zeigt diese Studie, dass eine hohe Proteomabdeckung mit einem Microflow-LC-MS/MS-System erreicht werden kann, das eine 1 mm ID × 150 mm Säule mit einer Flussrate von 50 μL/min und gekoppelt betrieben an ein Orbitrap HF-X-Massenspektrometer.
Die Ergebnisse zeigen die Identifizierung von 9 000 Proteinen aus 50 μg Proteinverdau aus Arabidopsis- Wurzeln, 7 500 aus Mausthymus und 7 300 aus menschlichen Brustkrebszellen in three h Analysezeit in einem einzigen Durchlauf. Der mit dem iBAQ-Ansatz gemessene dynamische Bereich der Proteinquantifizierung umfasste 5 Größenordnungen und die Wiederholungsanalyse zeigte, dass der mittlere Variationskoeffizient unter 20 % lag. Zusammen zeigt diese Studie, dass ssDDA durch μLC-MS/MS eine robuste Methode für umfassende und groß angelegte Proteomanalysen ist und in Zukunft auf schnellere Chromatographie- und datenunabhängige Akquisitionsansätze ausgeweitet werden kann.̀.
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human DNA-PKCS at AA range: 4030-4110
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human DNA-PKCS at AA range: 4030-4110
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human DNA-PKCS at AA range: 4030-4110
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2647
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2647
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2647
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2612
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2612
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2612
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2638
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2638
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2638
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2609
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2609
Description: A polyclonal antibody for detection of DNA-PKCS from Human. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of T2609
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2056
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2056
Description: A polyclonal antibody for detection of DNA-PKCS from Human, Mouse. This DNA-PKCS antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNA-PKCS around the non-phosphorylation site of S2056
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is unconjugated.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to APC .
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Biotin.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to FITC.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to HRP.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to PerCP.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to RPE .
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA3742-3755). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Streptavidin.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is unconjugated.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to APC .
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Biotin.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to FITC.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to HRP.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to PerCP.
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to RPE .
Description: A polyclonal antibody for DNA PKcs from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human DNA PKcs (AA4067-4081). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This DNA PKcs antibody is conjugated to Streptavidin.
